First, I may have bent the rules of this challenge. I am using "Contaminate with random cells" at liberty, to introduce completely new genomes into the substrate when other genome(s) are already living there. This breaks the restriction on only using it in the first 100h, but my thinking is that "Contaminate" is the quickest way to get a completely new organism, rather than just a derivative of an existing genome. This hasn't helped a lot so far--if a successful genome is already living on the plate, it is very hard for some random cells to get started as a new genome that can reproduce.
Also, I have been sometimes using manually inserted cells--after evolving a genome that can self-sustain, I may sterilize and add that genome again from an initial cell. But I have not touched any genome settings; nothing was designed, it is all the result of evolution from random cells, which I think is the point of this challenge. I think I've followed it in spirit, if not the letter.
So here are some of my attempts. First, the
Twin Tadpoles:
chaos-twin-tadpoles.substrate

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The swimmers (green) always hatch in pairs, from a double phagocyte egg (orange). The swimmer's phagocyte head makes new double-eggs. After the head produces a double-egg, the tail flies off by itself and dies. But sometimes, very rarely, their tail will split into a devorocyte. This devorocyte, if you can believe it,
also becomes an orange double-egg when it gets enough nutrients. So it's like a backup reproduction plan.
So far, they appear to survive indefinitely on this substrate, maintaining 200-300 cells.
Next, this unbelievably complicated photocyte-phagocyte organism:
chaos-elephant-man.substrate

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This thing uses, if I've counted them right,
14 modes in its life cycle, mostly phagocytes and photocytes with a sprinkling of buoycytes. The photocytes thrive in two colonies, where the light arc intersects the neutral buoyancy level. They generate phagocytes that may burst across huge areas of the plate when nutrients are plentiful. The photocyte colonies can survive on their own, and do not depend on the phagocyte portion of the life cycle--so even if some other organism eats most of the plate's nutrients, the photocyte colonies can stay alive. If either of the colonies dies, the other one can regenerate it. A later evolution of this genome has buoycytes allowing it to completely fill the top 1/3 of the plate.